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marco  (R&D Systems)


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    Structured Review

    R&D Systems marco
    ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil <t>recruitment</t> <t>(CD45</t> + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + <t>MARCO</t> + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).
    Marco, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+marco+antibody/Mouse+MARCO+Fluorescein-conjugated+Antibody/pmc12288981-259-30-33
    Average 90 stars, based on 10 article reviews
    marco - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Csk-mediated Src family kinase regulation dampens neutrophil infiltration during pulmonary infection"

    Article Title: Csk-mediated Src family kinase regulation dampens neutrophil infiltration during pulmonary infection

    Journal: JCI Insight

    doi: 10.1172/jci.insight.188323

    ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil recruitment (CD45 + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + MARCO + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).
    Figure Legend Snippet: ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil recruitment (CD45 + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + MARCO + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).

    Techniques Used: Injection, Cell Counting, Infection, Comparison

    Related Articles

    Control:

    Article Title: In vivo induction of activin A-producing alveolar macrophages supports the progression of lung cell carcinoma
    Article Snippet: .. Antibodies were obtained from the following sources: anti-CD45-PECy7 (1:50, clone 30-F11, #103114), anti-F4/80-BV421 (1:50, clone BM8, #123132), anti-CD11c-APC (1:50, clone N418, #117310), anti-CD11b-BV421 (1:50, clone M1/70, #101235), BV421-rat IgG2a, κ isotype control (1:50, clone RTK2758, #400535), and APC-human IgG1, κ isotype control (1:50, clone QA16A12, #403505) were from BioLegend (San Diego, CA); anti-Siglec-F-APC (1:50, clone REA798, #130-112-333), and anti-CD11b-APC (1:50, clone REA592, #130-113-802) were from Miltenyi Biotec (Bergisch Gladbach, NRW, Germany); anti-p-JNK (1:1000, #9251), anti-JNK (1:1000, #9252), anti-p-Erk1/2 (1:1000, #4370), anti-Erk1/2 (1:1000, #4695), anti-p-Smad2 (1:1000, #3108), anti-Smad2 (1:1000, #5339), anti-β-actin (1:1000, #5125), and anti-rabbit IgG, HRP-linked antibody (1:1000, #7074) were from Cell Signaling Technology (Danvers, MA); anti-Inhibin beta A (1:200, ab56057), and anti-TTF-1 (1:100, clone SP141, ab227652) were from Abcam (Cambridge, UK); anti-CD163 antibody (1:800, clone 10D6) was from Leica biosystems (Wetzlar, Germany); anti-MARCO antibody (1:500, clone 2359 A, MAB29561) was from R&D systems (Minneapolis, MN); AlexaFlour647 AffiniPure donkey anti-rabbit IgG (1:200, #711-605-152) was from Jackson Immuno Research (West Grove, PA); and anti-CD16/32 antibody (1:100, clone 2.4G2, #553141) was from BD Biosciences (San Jose, CA). .. Reagents were obtained from the following sources: Dulbecco’s Modified Eagle’s Medium (DMEM) (4.5 g/L glucose) with L-Gln, without sodium pyruvate (#08459-64), 1× phosphate-buffered saline (PBS) without Calcium and Magnesium (#14249-24), Hank’s balanced salt solution (HBSS) with calcium and magnesium, without phenol red (#09735-75), and RIPA buffer (#16488-34) were from Nacalai Tesque (Kyoto, Japan); Eagle’s Minimum Essential Medium (EMEM) (#30-2003) was from the American Type Culture Collection (ATCC, Manassas, VA); heat-inactivated fetal bovine serum (FBS; #F7524), penicillin/streptomycin (#P4333), and tamoxifen (#T5648) were from Sigma-Aldrich (St. Louis, MO); HEPES (#15630-080), and dispase (#17105-041) were from Gibco (Dublin, Ireland); c-Jun N-terminal kinase (JNK) inhibitor SP600125 (#S1460), TAK1 inhibitor Takinib (#S8663), and ALK4 inhibitor SB505124 (#S2186) were from Selleck Chemicals (Houston, TX); Toll-like receptor 4 (TLR4) inhibitor TAK-242 (#HT-11109), and MyD88 dimerization inhibitor ST2825 (#HY-50937) were from MedChemExpress (Monmouth Junction, NJ); recombinant human/mouse/rat activin A protein (#338-AC) was from R&D Systems (Minneapolis, MN); recombinant human follistatin (#120-13), and recombinant murine GM-CSF (#315-03) were from Peprotech (Cranbury, NJ); clodronate liposome (MKV100) and equivalent control liposome were from CosmoBio (Tokyo, Japan); Hoechst 33342 (#3570 invitrogen) was from Thermo Fisher Scientific (Waltham, MA); collagenase type IV (#LS004188) was from Worthington Biochemical Corporation (Lakewood, NJ); PhosSTOP (#4906845001), and EDTA-free Protease Inhibitor Cocktail (#4693159001) were from Roche (Basel, Switzerland).

    Article Title: In vivo induction of activin A-producing alveolar macrophages supports the progression of lung cell carcinoma.
    Article Snippet: .. Antibodies were obtained from the following sources: anti-CD45PECy7 (1:50, clone 30-F11, #103114), anti-F4/80-BV421 (1:50, clone BM8, #123132), anti-CD11c-APC (1:50, clone N418, #117310), anti-CD11bBV421 (1:50, clone M1/70, #101235), BV421-rat IgG2a, κ isotype control (1:50, clone RTK2758, #400535), and APC-human IgG1, κ isotype control (1:50, clone QA16A12, #403505) were from BioLegend (San Diego, CA); anti-Siglec-F-APC (1:50, clone REA798, #130-112-333), and anti-CD11b-APC (1:50, clone REA592, #130-113-802) were fromMiltenyi Biotec (Bergisch Gladbach, NRW, Germany); anti-p-JNK (1:1000, #9251), anti-JNK (1:1000, #9252), anti-p-Erk1/2 (1:1000, #4370), antiErk1/2 (1:1000, #4695), anti-p-Smad2 (1:1000, #3108), anti-Smad2 (1:1000, #5339), anti-β-actin (1:1000, #5125), and anti-rabbit IgG, HRPlinked antibody (1:1000, #7074) were from Cell Signaling Technology (Danvers, MA); anti-Inhibin beta A (1:200, ab56057), and anti-TTF-1 (1:100, clone SP141, ab227652) were from Abcam (Cambridge, UK); anti-CD163 antibody (1:800, clone 10D6) was from Leica biosystems (Wetzlar, Germany); anti-MARCO antibody (1:500, clone 2359A, MAB29561) was from R&D systems (Minneapolis, MN); AlexaFlour647 AffiniPure donkey anti-rabbit IgG (1:200, #711-605-152) was from Jackson Immuno Research (West Grove, PA); and anti-CD16/32 antibody (1:100, clone 2.4G2, #553141) was from BD Biosciences (San Jose, CA). .. Reagents were obtained from the following sources: Dulbecco’s Modified Eagle’sMedium (DMEM) (4.5 g/L glucose) with L-Gln, without sodium pyruvate (#08459-64), 1× phosphate-buffered saline (PBS) without Calcium and Magnesium (#14249-24), Hank’s balanced salt solution (HBSS) with calcium and magnesium, without phenol red (#09735-75), and RIPA buffer (#16488-34) were from Nacalai Tesque (Kyoto, Japan); Eagle’s Minimum Essential Medium (EMEM) (#30- 2003) was from the American Type Culture Collection (ATCC, Manassas, VA); heat-inactivated fetal bovine serum (FBS; #F7524), penicillin/ streptomycin (#P4333), and tamoxifen (#T5648) were from SigmaAldrich (St. Louis,MO);HEPES (#15630-080), and dispase (#17105-041) were from Gibco (Dublin, Ireland); c-Jun N-terminal kinase (JNK) inhibitor SP600125 (#S1460), TAK1 inhibitor Takinib (#S8663), and ALK4 inhibitor SB505124 (#S2186) were from Selleck Chemicals (Houston, TX); Toll-like receptor 4 (TLR4) inhibitor TAK-242 (#HT-11109), and MyD88 dimerization inhibitor ST2825 (#HY-50937) were from MedChemExpress (Monmouth Junction, NJ); recombinant human/mouse/ rat activin A protein (#338-AC) was from R&D Systems (Minneapolis, MN); recombinant human follistatin (#120-13), and recombinant murine GM-CSF (#315-03) were fromPeprotech (Cranbury, NJ); clodronate liposome (MKV100) and equivalent control liposome were from CosmoBio (Tokyo, Japan); Hoechst 33342 (#3570 invitrogen) was from Thermo Fisher Scientific (Waltham, MA); collagenase type IV (#LS004188) was from Worthington Biochemical Corporation (Lakewood, NJ); PhosSTOP (#4906845001), and EDTA-free Protease Inhibitor Cocktail (#4693159001) were from Roche (Basel, Switzerland).



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    R&D Systems marco
    ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil <t>recruitment</t> <t>(CD45</t> + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + <t>MARCO</t> + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).
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    Image Search Results


    ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil recruitment (CD45 + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + MARCO + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).

    Journal: JCI Insight

    Article Title: Csk-mediated Src family kinase regulation dampens neutrophil infiltration during pulmonary infection

    doi: 10.1172/jci.insight.188323

    Figure Lengend Snippet: ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil recruitment (CD45 + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + MARCO + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).

    Article Snippet: Alveolar macrophages were gated as CD45 + CD64 + F4/80 + MARCO + SiglecF hi using antibodies against CD45 (clone 30-F11, BioLegend), CD64 (clone X54-5/7.1, BioLegend), F4/80 (clone BM8, BioLegend), MARCO (clone 579511, R&D Systems), and SiglecF (clone S17007L, BioLegend).

    Techniques: Injection, Cell Counting, Infection, Comparison