marco (R&D Systems)
Structured Review

Marco, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+marco+antibody/Mouse+MARCO+Fluorescein-conjugated+Antibody/pmc12288981-259-30-33
Average 90 stars, based on 10 article reviews
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1) Product Images from "Csk-mediated Src family kinase regulation dampens neutrophil infiltration during pulmonary infection"
Article Title: Csk-mediated Src family kinase regulation dampens neutrophil infiltration during pulmonary infection
Journal: JCI Insight
doi: 10.1172/jci.insight.188323
Figure Legend Snippet: ( A – K ) Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice were subjected to K . pneumoniae intratracheal injection or sham surgery. After 24 hours, bacterial burden regarding the colony forming units (CFUs) in lung ( A ), bronchoalveolar fluid (BALF) ( B ), blood ( C ), and spleen ( D ) and neutrophil recruitment into the lungs ( E ) and BALF ( F ) were determined. ( G ) Neutrophil recruitment (CD45 + Gr-1 + LyB.2 + ) in Csk fl/fl Lyz2 wt/wt and Csk fl/fl Lyz2 cre/wt mice in the interstitial, intravascular, and BALF compartments following intratracheal instillation of K . pneumoniae . Cell count of monocytes (MO; CD45 + CD11b + CX3CR1 + Ly6C hi Ly6G – Gr-1 – ) ( H ), alveolar macrophages (AM; CD45 + CD64 + F4/80 + MARCO + SiglecF hi ) ( I ), natural killer cells (NK; CD45 + CD27 + CD335 + ) ( J ), and dendritic cells (DC; CD45 + CD27 – CD24 + CD11c + MHCII + ) ( K ) in lung tissue 24 hours after lung infection. n as indicated, mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by 1-way-ANOVA by Holm-Šídák multiple-comparison test ( A – D ), 1-way ANOVA with Tukey’s multiple-comparison test ( E , F , and H – K ), or 2-tailed Student’s t test ( G ).
Techniques Used: Injection, Cell Counting, Infection, Comparison
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Control:Article Title: In vivo induction of activin A-producing alveolar macrophages supports the progression of lung cell carcinoma Article Snippet: .. Antibodies were obtained from the following sources: anti-CD45-PECy7 (1:50, clone 30-F11, #103114), anti-F4/80-BV421 (1:50, clone BM8, #123132), anti-CD11c-APC (1:50, clone N418, #117310), anti-CD11b-BV421 (1:50, clone M1/70, #101235), BV421-rat IgG2a, κ isotype control (1:50, clone RTK2758, #400535), and APC-human IgG1, κ isotype control (1:50, clone QA16A12, #403505) were from BioLegend (San Diego, CA); anti-Siglec-F-APC (1:50, clone REA798, #130-112-333), and anti-CD11b-APC (1:50, clone REA592, #130-113-802) were from Miltenyi Biotec (Bergisch Gladbach, NRW, Germany); anti-p-JNK (1:1000, #9251), anti-JNK (1:1000, #9252), anti-p-Erk1/2 (1:1000, #4370), anti-Erk1/2 (1:1000, #4695), anti-p-Smad2 (1:1000, #3108), anti-Smad2 (1:1000, #5339), anti-β-actin (1:1000, #5125), and anti-rabbit IgG, HRP-linked antibody (1:1000, #7074) were from Cell Signaling Technology (Danvers, MA); anti-Inhibin beta A (1:200, ab56057), and anti-TTF-1 (1:100, clone SP141, ab227652) were from Abcam (Cambridge, UK); anti-CD163 antibody (1:800, clone 10D6) was from Leica biosystems (Wetzlar, Germany); Article Title: In vivo induction of activin A-producing alveolar macrophages supports the progression of lung cell carcinoma. Article Snippet: .. Antibodies were obtained from the following sources: anti-CD45PECy7 (1:50, clone 30-F11, #103114), anti-F4/80-BV421 (1:50, clone BM8, #123132), anti-CD11c-APC (1:50, clone N418, #117310), anti-CD11bBV421 (1:50, clone M1/70, #101235), BV421-rat IgG2a, κ isotype control (1:50, clone RTK2758, #400535), and APC-human IgG1, κ isotype control (1:50, clone QA16A12, #403505) were from BioLegend (San Diego, CA); anti-Siglec-F-APC (1:50, clone REA798, #130-112-333), and anti-CD11b-APC (1:50, clone REA592, #130-113-802) were fromMiltenyi Biotec (Bergisch Gladbach, NRW, Germany); anti-p-JNK (1:1000, #9251), anti-JNK (1:1000, #9252), anti-p-Erk1/2 (1:1000, #4370), antiErk1/2 (1:1000, #4695), anti-p-Smad2 (1:1000, #3108), anti-Smad2 (1:1000, #5339), anti-β-actin (1:1000, #5125), and anti-rabbit IgG, HRPlinked antibody (1:1000, #7074) were from Cell Signaling Technology (Danvers, MA); anti-Inhibin beta A (1:200, ab56057), and anti-TTF-1 (1:100, clone SP141, ab227652) were from Abcam (Cambridge, UK); anti-CD163 antibody (1:800, clone 10D6) was from Leica biosystems (Wetzlar, Germany); |